Table 1.
Primers used for DNA amplification, sequencing, and site-directed mutagenesis of atp genes of Mycobacteria species
| Primer name | Sequence (5′ to 3′) |
|---|---|
| atpBS | TGTAYTTCAGCCARGCSATGG |
| atpFAS | CCGTTSGGDABGAGGAAGTTG |
| atpBBKS | ATGACTGAGACCATCCTGGCC |
| atpBBKAS | AATCGCGCTCACTTCACCCAT |
| atpBABS_S | TTCGGGGTTGCGTTGTTCCAGG |
| atpEABS_AS | AACAATTGTGGGGTCCGCCAT |
| opeatpBKS1 | CGGGTGGTTTCCGCTCCATCA |
| opeatpBKAS2 | TGATGCG CTCCGCCTCGACGTC |
| opeatpBKS3 | AGCGCACCGTGTTGTGGAAG |
| opeatpBKAS4 | TAGATACGGCTCAGCACTTCGG |
| opeatpBKS5 | TGCGGTGGCTCGCCGCGGCGAAA |
| opeatpBKAS6 | TTTGAAACCGGCGGA CTCCGACGC |
| opeatpBKS7 | TCGGGCAGAAGGGAACTACCATCG |
| opeatpBKAS8 | GCTATTTCTTCTTCTTCGGCGCCGG |
| opeatpBKS9 | GCTTCGCGGCCACCGGTGGCGGCT |
| opeatpBKAS10 | ACCTAGCTTGGGCCTAGCGGGCTTC |
| opeatpBKS11 | ACGCTGATGGCAAACCGCGAGCG |
| opeatpBKAS12 | GCCAGCGTGGGCTGGTATCCCAC |
| opeatpBKS13 | CAAGACGTATTGCTGTTC ATCGACAACA |
| opeatpBKAS14 | ACAACGACGAGCACGACCATGCCGAT |
| atpEsmeLYGE | GGAATTCTTACTGAAGGCCAGGAGTGGC |
| atpEsmeLYGS | TCCCCCGGGATCTCGATCCCAACGCCATCA |
| atpEBKLYGE | GGAATTCTTACTTGACGGGTGTAGCGAA |
| atpEBKLYGS | TCCCCCGGGACCCCACTATCGCTGCCGGCG |
| pLYG204 | GGTTTCATCCCCGATCCGGAG |
| Primers used to mutate the M. smegmatis atpE gene | |
| atpEsmeI66MS | GCCGCGTACTTCATGAACCTGGCCTTCATGGCG |
| atpEsmeI66MAS | CGCCATGAAGGCCAGGTTCATGAAGTACGCGGC |
| atpEsmeD28GS | GCCGGTATCGGCGGCGGTATCGCGGGTAACGCG |
| atpEsmeD28GAS | CGCGTTACCCGCGATACCGCCGCCGATACCGGC |
| atpEsmeD28VS | GCCGGTATCGGCGTCGGTATCGCGGGTAACGCG |
| atpEsmeD28VAS | CGCGTTACCCGCGATACCGACGCCGATACCGGC |
| atpEsmeD28AS | GCCGGTATCGGCGCCGGTATCGCGGGTAACGCG |
| atpEsmeD28AAS | CGCGTTACCCGCGATACCGGCGCCGATACCGGC |
| atpEsmeE61DS | GTCGGTCTGGTGGACGCCGCGTACTTCATC |
| atpEsmeE61DAS | GATGAAGTACGCGGCGTCCACCAGACCGAC |
| atpEsmeA63PS | GGTCTGGTGGAAGCCCCGTACTTCATCAACCTGGCC |
| atpEsmeA63PAS | GGCCAGGTTGATGAAGTACGGGGCTTCCACCAGACC |
| atpEsmeL59VS | CCGTTCTTCATCACCGTCGGTGTGGTGGAAGCCGCGTA |
| atpEsmeL59VAS | TACGCGGCTTCCACCACACCGACGGTGATGAAGAACGG |
| atpEsmeA63MS | GGTCTGGTGGAAGCCATGTACTTCATCAACCTGGCC |
| atpEsmeA63MAS | GGCCAGGTTGATGAAGTACATGGCTTCCACCAGACC |
| Primers used to mutate the M. tuberculosis atpE gene | |
| atpEBKA63PS | GGTTTGGTTGAGGCGCCATACTTCATCAACCTGGCG |
| atpEBKA63PAS | CGCCAGGTTGATGAAGTATGGCGCCTCAACCAAACC |
| atpEBKE61DS | GTCGGTTTGGTTGACGCGGCATACTTCATCAAC |
| atpEBKE61DAS | GTTGATGAAGTATGCCGCGTCAACCAAACCGAC |
| atpEBKL59VS | CGTTCTTCATCACCGTCGGTGTGGTTGAGGCGGCATAC |
| atpEBKL59VAS | GTATGCCGCCTCAACCACACCGACGGTGATGAAGAACG |
| atpEBKI66MS | GCGGCATACTTCATGAACCTGGCGTTTATGGCG |
| atpEKI66MAS | CGCCATAAACGCCAGGTTCATGAAGTATGCCGC |
| atpEBKD28GS | GCCGGTATCGGTGGCGGTGTCGCCGGTAACGCG |
| atpEBKD28GAS | CGCGTTACCGGCGACACCGCCACCGATACCGGC |