Fig 4.
Potentiation of the anti-HIV-1 activity of ACV ProTides by ribavirin in MT-4 cell culture. MT-4 cells (105) infected with HIV-1LAI.04 were cultured for 4 days in the presence of ribavirin at concentrations ranging from 1 to 20 μM and ACV ProTides at concentrations ranging from 0.01 to 50 μM. HIV replication was evaluated by p24gag antigen release in culture medium at the end of the experiment using a bead-based assay. EC50s were calculated in an experiment in which two ACV ProTides (Cf 2648 and Cf2681) were tested against HIV-1LAI.04 as a function of different ribavirin concentrations.