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Insertion-inactivation of adeN. (A) Plasmid pAT524 (ΔadeN), possessing a truncated portion of adeN, underwent homologous recombination in the glnG-adeN-argF fragment, leading to (B) integration in the A. baumannii chromosome. (C) A second crossover event led to loss of the entire adeN and of the plasmid, resulting in a truncated adeN gene (D).
