Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s)a | Reference or source |
|---|---|---|
| Strains | ||
| A. baumannii | ||
| BM4587 | Wild-type clinical isolate | 4 |
| BM4666 | BM4587 adeNΔC582 spontaneous mutant obtained on cefotaxime | 4 |
| BM4667 | Wild-type clinical isolate | 4 |
| BM4668 | BM4667 adeNΔC582 spontaneous mutant obtained on tetracycline | 4 |
| BM4651 | BM4454 ΔadeABC | 6 |
| BM4669 | BM4651 adeNΔ258-651 spontaneous mutant obtained on tetracycline | This study |
| BM4685 | BM4587 ΔadeN | This study |
| E. coli | ||
| NEB5α | fhuA2 Δ(argF-lacZ)U169 phoA glnV44 Φ80(lacZ)ΔM15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 | New England Biolabs |
| CC118 | A(ara-leu) araD ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB argE(Am) recAl | 17 |
| CC118λpir | CC118 lysogenized with λpir | 11 |
| HB101 | supE44 hsdS20(rB− mB−) recA13 ara-14 leuB6 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 F−thi-1 | 14 |
| Plasmids | ||
| pCR2.1 | lacZα ColE1oriR Apr Kmr | Invitrogen |
| pCRBlunt | Kmr Zeocinr ColE1oriR lacZα ccdB | Invitrogen |
| pUC18 | Tra− Mob− AprlacZα | 26 |
| pAT801 | Apr Tcr | 10 |
| pAT522 | 702-bp adeN fragment of BM4587 cloned in pAT801 | This study |
| pAT523 | 701-bp adeNΔC582 fragment of BM4666 cloned in pAT801 | This study |
| pKNG101 | Suicide vector, sacB Smr | 13 |
| pRK2013 | Helper plasmid; ColE1oriR Tra Mob Kmr | 8 |
| pAT524 | 1,154-bp truncated adeN cloned in pKNG101 | This study |
a
Ap, ampicillin; Km, kanamycin; Mob, mobilizable; r, resistant; Sm, streptomycin; Tc, tetracycline; Tra, self-transferable.