Table 2.

Oligonucleotides

Primer	Sequence (5′ → 3′)a	Positionb
adeN1	ggatccGCTGTTAGGTTGGGGTCGTA	−387 to −368
adeN2c	ggagatgTTTTTGAACCACCAGCATGA	172–153
adeN3d	tcaaaaaCATCTCCCGATCATTCCTCT	497–516
adeN4	gggcccCGTGACCAAAAGTACGAATCA	1091–1071
adeN5v	TCGTGAGGTTTATCCGGAAG	−645 to −636
adeN6v	TGCATTTCAACCAGAGCAAA	264–283
adeN7v	GAAACCCATCATGTGTGCAG	1294–1275
adeN9	AACGACGGCTAACACTGCTT	−11 to −30
adeNM3	TGCATGATCCAGTCCTTGAG	2–21
adeNM4	ACCACCAGCATGATTAACGA	165–146
adeNAYE3	CATTTCAAAATGCAAAAGAATT	674–653
adeNAYE4	GCAGTGTTAGCCGTCGTTAT	−28 to −9
adeNAYE5	AAAACATTTCACGGCGATAA	247–228
AdeNAYE7	TTATCGCCGTGAAATGTTTT	228–247
adeNAYE8	ATTTTGGACATCCAGAGCAC	444–425
TailAdeN1	TCATGCTGGTGGTTCAAAAA	−122 to −102
TailAdeN2	ACACGCCGCGGTATAGAAC	51–70
TailAdeN3	TTTTCCCCAAAACTATGCTCT	152–171
adeJ For	GGTCATTAATATCTTTGGC	1143–1161
adeJ Rev	GGTACGAATACCGCTGTCA	1364–1346
rpoB For	TCCGCACGTAAAGTAGGAAC	2114–2095
rpoB Rev	ATGCCGCCTGAAAAAGTAAC	1959–1979

^aThe restriction sites introduced in the oligonucleotides are underlined.

^bCoordinates refer to the first base of the gene.

^cThe first 7 nucleotides are complementary to those of adeN3 indicated in italics.

^dThe first 7 nucleotides are complementary to those of adeN2 indicated in italics.