Table 3.

Primers used in PCR and sequencing

Target	Primer	Sequence (5′ to 3′)	Specificity	Application
gyrB	gyrB-Uu-F	TAGAGAATGAAGTTATAGTTG	U. urealyticum	PCR/sequencing
	gyrB-Uu-R	CTTCTTTTGTAATTGATTGT	U. urealyticum	PCR/sequencing
	gyrB_Uu_743F	ACGGTGAAGAAGAAAAAGA	U. urealyticum	Sequencing
	gyrB_Uu_1388R	AATGGTAAAATTGCTTGGAA	U. urealyticum	Sequencing
gyrA	gyrA_386F	TTGATGGTGATAGTGCAG	U. urealyticum	Sequencing
	gyrA_Uu_1722R	TAGTAAGTCAGTGTGGGT	U. urealyticum	Sequencing
parE	parE-Uu-F	TGATTGAGTAAAGATTGGAA	U. urealyticum	PCR/sequencing
	parE_Uu_ 1104R	GGCTTGTGTTTTATTTTCTT	U. urealyticum	Sequencing
	parE_Uu_1193F	TAAAGAAGAACGTGGAATGG	U. urealyticum	Sequencing
	parE_Uu_R2	CGCTCATGGCATACAAAATA	U. urealyticum	PCR/sequencing
	parE_CF	CAACATGAAATTGGTCATTTAGATGC	Both speciesa	PCR/sequencing
	parE_CR	GCATAACTTTCACCAACAATGTTGTCT	Both species	PCR/sequencing
	parE_809CF	CTGTGAAAACTAGTGAAGGTGGTGT	Both species	Sequencing
	parE_1047CR	TACTGCTTCATTTGCTTCTGGTGTG	Both species	Sequencing
parC	parC-Uu-F	TGGGTAATGATGTAAGTATT	U. urealyticum	PCR/sequencing
	parC-Uu-R	TCATTTAGTTTTGGTGTATAG	U. urealyticum	PCR/sequencing
	parC_Uu_1541R	CCATCACGACTAATAACTAAA	U. urealyticum	Sequencing
	parC_Uu_1338F	TCAACAACAAGCTAAAACAC	U. urealyticum	Sequencing
	parC_CF	GGGTAATGACGTAAGTATTCGA	Both species	PCR/sequencing
	parC_856CR	CATCACGAACTTCGATTAATCCAGC	Both species	Sequencing
	parC_693CF	TGCTCACAAAACTGGTGAAGGT	Both species	Sequencing
	parC_1738CR	GTTCACCAACATCTTTTCACCGCAT	Both species	Sequencing
	parC_1375CF	TCACTTCAAGTTCGCAAAAATT	Both species	Sequencing
	parC_2269CR	CAACTAAAAAAGCTGCTAAAAC	Both species	Sequencing
	parC_2093CF	ATGAAATTAAAACCAAATGATGA	Both species	Sequencing
	parC_2672CR	TTTAAAACAACGTTGGCATAAATT	Both species	PCR/sequencing

^aUreaplasma parvum and Ureaplasma urealyticum.