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. Author manuscript; available in PMC: 2012 May 7.
Published in final edited form as: Cell Rep. 2012 Mar;1(4):325–333. doi: 10.1016/j.celrep.2012.02.010

Figure 3. Inducible Suppression of eIF4E Impairs eIF4F Levels, Selectively Blocks Protein Synthesis, and Affects Production of Cyclin D1, Mcl-1, and Myc.

Figure 3

A. Relative abundance of the eIF4F complex in B220+ cells from mice of the indicated genotype. B. Protein synthesis rates in B220+ cells assessed by [35S]methionine incorporation and TCA precipitation. Error bars denote SEM; n=3. C. Western blot analysis of eIF4E responsive targets in B220+ cells isolated from 6 wk old mice. Cells were isolated from 4E.389/rtTA (lane 1), 4E.389/rtTA/Eμ-Myc (lane 2), and 4 independent C57BL/6 (lanes 3–6) or Eμ-Myc (lanes 7–10) mice. D. Western blot analysis showing reductions in Mcl-1, Cyclin D1, and Myc levels upon suppression of eIF4E in B220+ cells in vivo. E. qRT-PCR quantitation of the indicated mRNA levels from B220+ splenocytes. N=6; *, p = 0.003. F. Western blot analysis indicating levels of eIF4E targets upon eIF4E suppression in B220+ cells.