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Measured versus expected JAK2 V617F percentage. Serial dilutions of UKE-1 cells (supposedly homozygous for mutant JAK2 V617F) in HL60 cells (homozygous for wild-type JAK2) were made and the percentage of JAK2 V617F in each dilution was quantified using the JAK2 Mutaquant Ipsogen kit. For each dilution, the measured JAK2 V617F percentage was significantly greater than the theoretical ratio shown by the dotted line (P=0.002).
