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. 1988 Jan 25;16(2):413–424. doi: 10.1093/nar/16.2.413

Mechanism of autonomous control of the Escherichia coli F plasmid: purification and characterization of the repE gene product.

L Masson 1, D S Ray 1
PMCID: PMC334669  PMID: 3277161

Abstract

E protein, the 29 Kd repE gene product, is essential for the replication of the Escherichia coli F plasmid. The repE gene has been cloned and expressed in an inducible ATG-fusion vector, and the protein product has been purified to homogeneity. The purified E protein is present as a dimer in solution and binds specifically to both the 19-bp direct repeats (incB) found within the mini-F origin of replication ori2 and the repE operator DNA. Examination of the amino acid sequence of E protein revealed a consensus sequence for DNA binding.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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