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. 2001 May 15;98(11):6325–6329. doi: 10.1073/pnas.101136898

Figure 2.

Figure 2

Treatment of MHC Class II −/− mice with agonistic antibodies to CD40 does not increase virus-specific antibody responses or IFN-γ production. MHC Class II −/− or wild-type mice were infected with MHV-68 and treated with anti-CD40 or an isotype control antibody as described in the legend to Fig. 1. Mice were killed 50 days after infection. (A) Virus-specific serum antibody titers were determined by ELISA. The secondary antibody detects IgM + IgG + IgA (heavy and light chains). Data are expressed as mean serum antibody titers for 8–10 individual mice from three separate experiments. Two to four mice were sampled in each experiment. (B) IFN-γ production. Splenocytes were restimulated in vitro with virus-infected APCs, and IFN-γ in the supernatants was determined by ELISA. Data are expressed as mean IFN-γ concentrations + SEM for 4–6 mice from two separate experiments. Peak values (which were usually attained after 72-h culture) are reported. Duplicate cultures from two to three mice were sampled in each experiment. The detection limit was 13.7 pg IFN-γ/ml.