ApoE levels in (A) medium and (B) cultured macrophages (average of 2 independent experiments, each performed in triplicate, representative western blot and immunostain, scale bar=50µm). (C) Apoe mRNA (n=4–8) and (D) activated populations of macrophages isolated from mice fed a HCD for 4 weeks (n=9–10 females and males grouped). Mean±SEM, ***p<0.001 versus WT; #p<0.05, ###p<0.001 ApoeTh/h versus ApoeRh/h