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. 2012 May 7;7(5):e35919. doi: 10.1371/journal.pone.0035919

Figure 2. Lipopolysacharide (LPS) and unconjugated bilirubin (UCB) inhibit P-glycoprotein (P-gp) activity in brain microvascular endothelial cells.

Figure 2

To evaluate if P-gp is functioning properly, it was measured the protein’s ability to remove the substrate rhodamine 123 (R123). After treatment, cells were incubated for 30 min with 10 µM R123. Total protein extracts were collected, R123 accumulation fluorescent emission was determined and results were expressed per mg of protein. Values presented are means ± S.E.M., from at least four independent experiments performed in duplicate. *P<0.05, **P<0.01 and ***P<0.001 vs. respective control; § P<0.05 vs. LPS at the same time-point; # P<0.05 from 4 h.