Figure 4. Lipopolysaccharide (LPS) and unconjugated bilirubin (UCB) disrupt ultrastructure of brain endothelial cells.

Cells were treated with no addition (control) (A,D,E), LPS (B,F,G) or UCB (C,H,I) and were analyzed by transmission electron microscopy. Black arrows, tight intercellular junctions; grey arrows, disruption of the plasma membrane; arrowheads, invaginations of the plasma membrane; a, apoptotic cells bodies; m, mitochondria; N, cell nuclei; RER, rough endoplasmic reticulum; v, vacuole. Representative results from one of four independent experiments are shown.