Figure 9. Lipopolysaccharide (LPS) and unconjugated bilirubin (UCB) alter expression of claudin-5 in brain endothelial cells.

Cells, either in mono-culture or co-cultured with astrocytes, were fixed and immunostained with an antibody against claudin-5 to evaluate its cellular localization, pattern of expression and integrity of the monolayer. Disruption of the monolayer with gaps between endothelial cells (*) and alterations in protein patterns (arrowheads) are indicated. Representative results from one of two independent experiments are shown. Scale bar, 20 µm.