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. 2012 May 7;7(5):e36754. doi: 10.1371/journal.pone.0036754

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Leenders et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(a) schematic overview of MYOCD, MRTF-A and MRTF-B. All the proteins share a SRF binding and a (potential) KLF15 binding domain. (b) KLF15 represses MRTF-A and MRTF-B mediated activation of the SRF responsive −505 Sm22 luciferase reporter. (c) KLF15 represses MRTF-A and MRTF-B mediated activation of the SRF responsive −638 ANF luciferase reporter. (d) A GST-pulldown assay using in vitro translated ³⁵S labeled MRTF-A and MRTF-B and GST-fused KLF15 shows a direct interaction between KLF15 and MRTF-A and –B. (e) GST pulldown assays using ³⁵S labeled SRF and GST fused KLF15 shows no interaction between KLF15 and SRF. (f) KLF binding site (CACCC) in the −505 Sm22 reporter. This bindingsite is mutated to a TGTTT site. (g) deletion of the KLF binding site in the −505 Sm22 reporter does not affect the repressive effect of KLF15.