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. 2012 May 7;7(5):e36152. doi: 10.1371/journal.pone.0036152

Figure 7. In vitro plate confrontation assays of WT, ΔEng18B and ΔEng18B+ T. atroviride strains.

Figure 7

(A) Plate confrontation against B. cinerea. Agar plugs of T. atroviride (right side in the plate) and B. cinerea (left side in the plate) were inoculated on opposite sides in 9 cm SMS agar plates and incubated at 25°C in darkness. The experiment was performed in three replicates and photographs of representative plates were taken 15 days post inoculation. (B and D) Secretion assay of B. cinerea. Agar plugs of B. cinerea was inoculated on SMS agar plates covered with cellophane and incubated at 25°C in darkness. After reaching the same diameter the colony was removed together with the cellophane disc and the plates re-inoculated with a T. atroviride WT, ΔEng18B or ΔEng18B+ agar plug and incubated at 25°C in darkness. Growth rate was calculated from data recorded 5 days post inoculation. The experiment was performed in three replicates and photographs of representative plates were taken 5 days post inoculation. (C and E) Secretion assay of WT, ΔEng18B and ΔEng18B+ T. atroviride strains. Agar plugs of T. atroviride WT, ΔEng18B or ΔEng18B+ strains were inoculated on SMS agar plates covered with cellophane and incubated at 25°C in darkness. After reaching the same diameter the colony was removed together with the cellophane disc and the plates re-inoculated with a B. cinerea agar plug and incubated at 25°C in darkness. Growth rate was calculated from data recorded 5 days post inoculation. The experiment was performed in three replicates and photographs of representative plates were taken 5 days post inoculation. Different letters indicate statistically significant differences (P≤0.05) within experiments.