Table 1. Calculated Effective concentrations EC₅₀ for E2, EE2, E1 and Genistein in transgenic cyp19a1b-GFP zebrafish line based on measurement of either cyp19a1b or GFP gene expression by PCR and by image analysis.

Compound	Method	EC50 (nM)	±CI 95
E2	cyp19a1b mRNA	2.8	2,61−2,99
	GFP mRNA	4.1	1.55−8.16
	Image analysis	0.5	0.45−0.64
EE2	cyp19a1b mRNA	0.04	0.031−0.051
	GFP mRNA	0.02	0.012−0.028
	Image analysis	0.01	0.01−0.012
E1	cyp19a1b mRNA	2.3	2.14−1.81
	GFP mRNA	1.3	0.88−2.15
	Image analysis	2.4	2.04−2.5
Genistein	cyp19a1b mRNA	3545	3052−3556
	GFP mRNA	2466	1172−19261
	Image analysis	2166	1872−2254

Results are expressed as mean ± confidence interval at 95% (CI 95).