Table 1. Calculated Effective concentrations EC50 for E2, EE2, E1 and Genistein in transgenic cyp19a1b-GFP zebrafish line based on measurement of either cyp19a1b or GFP gene expression by PCR and by image analysis.
Compound | Method | EC50 (nM) | ±CI 95 |
E2 | cyp19a1b mRNA | 2.8 | 2,61−2,99 |
GFP mRNA | 4.1 | 1.55−8.16 | |
Image analysis | 0.5 | 0.45−0.64 | |
EE2 | cyp19a1b mRNA | 0.04 | 0.031−0.051 |
GFP mRNA | 0.02 | 0.012−0.028 | |
Image analysis | 0.01 | 0.01−0.012 | |
E1 | cyp19a1b mRNA | 2.3 | 2.14−1.81 |
GFP mRNA | 1.3 | 0.88−2.15 | |
Image analysis | 2.4 | 2.04−2.5 | |
Genistein | cyp19a1b mRNA | 3545 | 3052−3556 |
GFP mRNA | 2466 | 1172−19261 | |
Image analysis | 2166 | 1872−2254 |
Results are expressed as mean ± confidence interval at 95% (CI 95).