Table 2. Effective concentrations (EC₅₀), maximum fold of induction measured above solvent control and relative estrogenic potencies (REP) of various compounds belonging to different chemical families.

	Substance	EC50 (nM)	SD	Max. fold induction	SD	CV (%)	REP	n
Synthetic estrogens	EE2	0.013	0.004	18	6.6	31.4	36.6	6
	HEX	0.012	0.002	24	2.1	18.6	39.1	3
	DES	0.01	0.004	22.8	1.7	36.6	45.8	3
Natural estrogens	E1	1.3	0.23	18.8	6.4	18.1	0.36	3
	E2	0.48	0.27	16.4	8.5	57.4	1	4
	E3	83.9	22.9	8.6	0.5	27.3	0.01	4
	4tOP	595	131.5	11.0	4.2	45.0	8.01E-04	3
Alkylphenols	4tPP	2541	503	10.0	3.5	19.8	1.88E-04	4
	4NPmix	406	94.4	9.3	0.3	20.9	1.17E-03	4
	4-n-NP	n.e.					-	2
	BPA	3303	933	11.5	0.5	28.3	1.44E-04	5
bisphenol	Zearalenone	16	3.46	20	1.0	18.5	0.030	3
Phyto & myco-estrogens	α-Zearalanol	>500		6.9	0.1	7.4	-	2
	α-Zearalenol	>500		5.5	1.0	9.7	-	2
	β-Zearalenol	>500		4	0.8	9.7	-	2
	Genistein	2501	6.1	8.1	0.3	0.2	1.91E-04	3
	Daidzein	n.e.					-	2
Pesticides	op’DDT	257	25.4	11.4	0.6	9.9	1.86E-03	3
	MXC	85	19.7	9.0	1.5	23.3	5.63E-03	3
	HPTE	477	49.2	7.4	1.6	10.3	9.99E-04	4
	Chlordecone	n.e.	-	-	-	-	-	2
	Endosulfan	n.e.	-	-	-	-	-	2
Androgens	Testosterone	1031	313	11.3	2.3	30	4.63E-04	3
	17α-MT	35.4		19.0	4.5		0.013	2
	11-Ketotesterone	n.e.	-	-	-	-	-	2
	DHT	2003	697	20.9	3.7	35	2.38E-04	3
	17β-trenbolone	508		13.3	4.9		9.38E-04	2
	R1881	108		8.2	0,47		-	2
Progestagens	Norethindrone	9.01	0.58	20.1	4.2	6.4	0.053	3
	D(−)Norgestrel	77.1	17.63	19.0	3.3	22.8	6.19E-03	2
	Progesterone	n.e
Other compounds	Spironolactone	n.e
	Dexamethasone	n.e.
	Rifampicine	n.e.
	Corticosterone	n.e.
	TCDD	n.e.
	BaP	n.e.
	BaA	n.e.
	diBaA	n.e.
	Chrysène	n.e.
	Benzophenone	n.e.
	EtOH	n.e.
	MetOH	n.e.
	KMnO4	n.e.

Results are expressed as mean ± standard deviation (SD).

N = number of independent experiments, n.e.: no effect, CV(%) = coefficient of variation inter-assay for EC₅₀. For each experiment, 10–15 transgenic zebrafish embryos were analyzed per condition.