Table 2. Effective concentrations (EC50), maximum fold of induction measured above solvent control and relative estrogenic potencies (REP) of various compounds belonging to different chemical families.
Substance | EC50 (nM) | SD | Max. fold induction | SD | CV (%) | REP | n | |
Synthetic estrogens | EE2 | 0.013 | 0.004 | 18 | 6.6 | 31.4 | 36.6 | 6 |
HEX | 0.012 | 0.002 | 24 | 2.1 | 18.6 | 39.1 | 3 | |
DES | 0.01 | 0.004 | 22.8 | 1.7 | 36.6 | 45.8 | 3 | |
Natural estrogens | E1 | 1.3 | 0.23 | 18.8 | 6.4 | 18.1 | 0.36 | 3 |
E2 | 0.48 | 0.27 | 16.4 | 8.5 | 57.4 | 1 | 4 | |
E3 | 83.9 | 22.9 | 8.6 | 0.5 | 27.3 | 0.01 | 4 | |
4tOP | 595 | 131.5 | 11.0 | 4.2 | 45.0 | 8.01E-04 | 3 | |
Alkylphenols | 4tPP | 2541 | 503 | 10.0 | 3.5 | 19.8 | 1.88E-04 | 4 |
4NPmix | 406 | 94.4 | 9.3 | 0.3 | 20.9 | 1.17E-03 | 4 | |
4-n-NP | n.e. | - | 2 | |||||
BPA | 3303 | 933 | 11.5 | 0.5 | 28.3 | 1.44E-04 | 5 | |
bisphenol | Zearalenone | 16 | 3.46 | 20 | 1.0 | 18.5 | 0.030 | 3 |
Phyto & myco-estrogens | α-Zearalanol | >500 | 6.9 | 0.1 | 7.4 | - | 2 | |
α-Zearalenol | >500 | 5.5 | 1.0 | 9.7 | - | 2 | ||
β-Zearalenol | >500 | 4 | 0.8 | 9.7 | - | 2 | ||
Genistein | 2501 | 6.1 | 8.1 | 0.3 | 0.2 | 1.91E-04 | 3 | |
Daidzein | n.e. | - | 2 | |||||
Pesticides | op’DDT | 257 | 25.4 | 11.4 | 0.6 | 9.9 | 1.86E-03 | 3 |
MXC | 85 | 19.7 | 9.0 | 1.5 | 23.3 | 5.63E-03 | 3 | |
HPTE | 477 | 49.2 | 7.4 | 1.6 | 10.3 | 9.99E-04 | 4 | |
Chlordecone | n.e. | - | - | - | - | - | 2 | |
Endosulfan | n.e. | - | - | - | - | - | 2 | |
Androgens | Testosterone | 1031 | 313 | 11.3 | 2.3 | 30 | 4.63E-04 | 3 |
17α-MT | 35.4 | 19.0 | 4.5 | 0.013 | 2 | |||
11-Ketotesterone | n.e. | - | - | - | - | - | 2 | |
DHT | 2003 | 697 | 20.9 | 3.7 | 35 | 2.38E-04 | 3 | |
17β-trenbolone | 508 | 13.3 | 4.9 | 9.38E-04 | 2 | |||
R1881 | 108 | 8.2 | 0,47 | - | 2 | |||
Progestagens | Norethindrone | 9.01 | 0.58 | 20.1 | 4.2 | 6.4 | 0.053 | 3 |
D(−)Norgestrel | 77.1 | 17.63 | 19.0 | 3.3 | 22.8 | 6.19E-03 | 2 | |
Progesterone | n.e | |||||||
Other compounds | Spironolactone | n.e | ||||||
Dexamethasone | n.e. | |||||||
Rifampicine | n.e. | |||||||
Corticosterone | n.e. | |||||||
TCDD | n.e. | |||||||
BaP | n.e. | |||||||
BaA | n.e. | |||||||
diBaA | n.e. | |||||||
Chrysène | n.e. | |||||||
Benzophenone | n.e. | |||||||
EtOH | n.e. | |||||||
MetOH | n.e. | |||||||
KMnO4 | n.e. |
Results are expressed as mean ± standard deviation (SD).
N = number of independent experiments, n.e.: no effect, CV(%) = coefficient of variation inter-assay for EC50. For each experiment, 10–15 transgenic zebrafish embryos were analyzed per condition.