Table 1. Abscess size and colonization of catheters according to dose of three Staphylococcus epidermidis strains in inbred C57BL/6JCrl , outbred Crl:CD1(ICR), and inbred BALB/cAnNCrl mice#.
Abscess size (mm2), strain of mice, and bacterial dose (CFUs*) | Colonization of catheters (no. of CFUs ± SEM‡), strain of mice, and bacterial dose (CFUs) | |||||||||||
Bacteria | C57BL/6JCrl | Crl:CD1(ICR) | BALB/cAnNCrl | C57BL/6JCrl | Crl:CD1(ICR) | BALB/cAnNCrl | ||||||
108 | 109 | 108 | 109 | 108 | 109 | 108 | 109 | 108 | 109 | 108 | 109 | |
S. epidermidis WT§ | 12±1a | 41.8±3a | 10±2.3a | 39.8±3.5a | 22.5±5.4b | 63.8±4.7a | 286±97a | 1,235±60a | 5,993±1,937a | 9,900±3,580a | 2,468±1,910a | 9,960±3,340a |
S. epidermidis hemB £ | 0a | 16.5±1a | 0a | 7.8±1.1b | 0a | 28.3±5.5c | 56±46a | 0a | 1,215±454a | 9,540±3,659b | 4,195±3,425a | 3,305±2,330a , b |
S. epidermidis CM+ | 27±2.7a | 26±4a | 8.75±1.6a | 55±2.3b | 19.5±6.5a | 68.8±5.5b | 3,917±1,497a | 3,220±2,161a | 807±536a | 404±39a | 5,800±2,333a | 11,524±8,168a |
four 8–12 week old male mice per bacterial- dose and phenotype were used.
CFUs: colony-forming unit,
SEM: standard error of the mean,
WT: wild type,
hemB: hemB knock-out mutant with small-colony-variant phenotype,
CM: complemented mutant of S. epidermidis hemB.
Values with different superscripts within a row, assigned to the same infectious dose, and separated between abscess size and colonization vary significantly (p<0.05; Student's unpaired t-test).