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Sucrose density gradient sedimentation profiles of ERα and ERβ. (a) ERα was from the cytosol of MCF7 cells (⧫) and uteri of ovariectomized rats (▵). ERβ (○) was 503 aa from extracts of SF9 cells expressing this protein. (b) Prostate cytosols were from rats that were 6 (○), 8 (◊), 10 (●), and 12 (▵) weeks of age. (c) Prostate cytosol was from 9-week-old rats. Tritiated ligands: a and b, 10 nM E2; c, 5 nM tritiated 3βAdiol with (−) and without (×) 5 nM radioinert E2.
