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Protocol used with multiple lectin affinity chromatography for the study of glycoproteins in serum. Four techniques were used in this separation: immunodepletion, glycoprotein fractionation, isoelectric focusing (IEF) fractionation using a digital proteome chip (dPC), and reversed-phase liquid chromatography in conjunction with mass spectrometry (LC-MS). Reproduced with permission from Ref. [55].
