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Examples of (a) a zonal elution competition study with R-warfarin and gliclazide as a competing agent on an HSA column and (b) a frontal analysis experiment for gliclazide applied to an HSA column, with data being fitted to a two-site binding model. Terms: k, retention factor; mL,app, moles of applied target that are needed to reach the mean position of the breakthrough curve at a given concentration of the target. Adapted with permission from Ref. [121].
