FIG. 4.

The head bobber locus on chromosome 7 contains a duplicated fragment of chromosome X. A Chromosome-7- (133J5, red) and chromosome-X (436I3, green)-specific BAC clones are seen to hybridize to their respective chromosomal loci in wild-type (wt) male spleen cells, whereas in female hb/hb cells, the chromosome 7 BAC clone (436J3, red) also co-localizes with the chromosome X (133J5, green) clone (B). Both chromosome 7 (green in C) and chromosome X (green in D) BAC clones co-localize with the p88 vector on the chromosome 7 from hb/hb spleen cells. E Splice variants of the Reps2 gene are appropriately expressed in hb/hb ears as measured by RT-PCR (see “Methods” for primers used). Blast hit, sequence comparison location. F Diagram of the head bobber locus (red line) showing nucleotide positions of the transgene integration site and junctions on chromosome 7 having three copies of the transgene (green boxes, neo; blue boxes, β-actin cassette). chr7, chromosome 7; chrX, chromosome X.