Effect of HN polypeptides on neuronal cell death by FAD genes.
(A) F11 cells were transfected with V642I-APP cDNA and
were treated with various concentrations of sHN, sHNG, sHNA, the dimer
form of sHN through C8 (C8-C8), and sHN whose C-terminal KRRA was
replaced with AAAA (KRRA to AAAA). Seventy-two hours after
transfection, cell mortality was measured by Trypan blue exclusion
assay. (B) F11 cells were transfected with M146L-PS1 or
N141I-PS2 cDNA, and were treated with various concentrations of sHN,
sHNG (S14G), or sHNA (C8A). Seventy-two hours after transfection, cell
mortality was similarly measured.