Fig. 3.

T. gondii SAG2 typing of the vegetables samples. b Sau3AI digestions of 5′ SAG2 amplification products loaded onto a 2% gel agarose. b CfoI digestions of 3′ SAG2 amplification products loaded onto a similar gel. Line 1, molecular weight marker (pUC19 DNA/MspI (HpaII) Marker, 23, Fermentas, Lithuania). Line 2, positive control (RH strain, type I). Samples loaded in lines 3, 4, 5, 6, 7 and 8 appear to be SAG2 type I (no digestion). Samples loaded in lines 9 and 10 contain SAG2 type II parasite (digestion with CfoI endonuclease and lack of digestion with Sau3AI restriction enzyme)