Table 1.
Results of the Toxoplasma gondii oocysts detection in fruits and vegetables samples taken from different places (cities: Gdansk, Gdynia, Sopot and villages: Olesno, Kostkowo, Chynow). I: genotype SAG2I, II: genotype SAG2II, ND: genotype not determined, +: positive result of amplification, −: negative result of amplification, N: initial copy number determined using real-time qualitative polymerase chain reaction (QPCR), OCS: approximate oocysts charge of sample
| Sample no. | Sampling place | Sample type | Result of real-time PCR (B1 gene) | Result of PCR (SAG2 gene) | Genotype | N | OCS | |
|---|---|---|---|---|---|---|---|---|
| 3′ end | 5′ end | |||||||
| 9 | Gdansk (bazaar) | Carrot | + | + | + | II | 98.3 | 3.5 |
| 19 | Gdansk (bazaar) | Radish | + | + | + | I | 121 | 4.3 |
| 79 | Gdynia (greengrocer) | Radish | + | + | + | I | 123.4 | 4.4 |
| 95 | Gdansk (bazaar) | Carrot | + | + | + | I | 141.3 | 5.0 |
| 133 | Gdansk (bazaar) | Carrot | + | − | + | ND | 224.4 | 8.0 |
| 134 | Gdansk (bazaar) | Carrot | + | + | + | I | 1580 | 5.6 |
| 144 | Gdansk (bazaar) | Radish | + | + | + | II | 76.4 | 2.7 |
| 147 | Sopot (greengrocer) | Lettuce | + | − | − | ND | 30.8 | 1.1 |
| 151 | Sopot (greengrocer) | Radish | + | − | − | ND | 35.5 | 1.3 |
| 139 | Gdynia(greengrocer) | Lettuce | + | − | − | ND | 49.4 | 1.8 |
| 137 | Sopot (greengrocer) | Radish | + | − | − | ND | 58.7 | 2.1 |
| 138 | Sopot (greengrocer) | Lettuce | + | − | − | ND | 59.4 | 2.1 |
| 143 | Gdynia (greengrocer) | Radish | + | − | − | ND | 48.2 | 1.7 |
| 132 | Gdansk (bazar) | Radish | + | − | − | ND | 27.8 | 1.0 |
| 185 | Kostkowo (farm) | Lettuce | + | + | + | I | 138.5 | 4.9 |
| 187 | Kostkowo (farm) | Carrot | + | + | + | I | 552.2 | 19.7 |
| 205 | Olesno (farm) | Carrot | + | + | − | ND | 58.7 | 2.1 |
| 208 | Olesno (farm) | Carrot | + | + | − | ND | 112.1 | 4.0 |
| 191 | Chynow (farm) | Carrot | + | − | − | ND | 403 | 1.4 |
| 192 | Chynow (farm) | Lettuce | + | − | − | ND | 30.8 | 1.1 |
| 182 | Kostkowo (farm) | Carrot | + | − | − | ND | 50.8 | 1.8 |