Figure 2.

Inhibition of uPA activity does not prevent LPA-induced E-cadherin junction disruption. OVCA429 cells were treated with or without LPA (30 μM), as indicated, for 18 hours in the presence or absence of the uPA inhibitor designated uPA Stop (2.5 μM), as indicated and processed for immunofluorescence staining for E-cadherin using anti-E-cadherin ectodomain antibody (1 : 300) and Alexa Fluor 488-conjugated secondary antibody (1 : 500; green). Blue-DAPI-stained nuclei.