Figure 3.

LPA induces MMP-dependent E-cadherin ectodomain shedding. (a, b) OVCA429 or (c, d) OVCA433 cells were treated with LPA (30 μM) in the presence or absence of the broad-spectrum MMP inhibitor GM6001 (25 μM) as indicated for 24 hours. Conditioned media were subjected to immunoprecipitation with anti-E-cadherin antibodies, and precipitates were western blotted with a second E-cadherin antibody (Zymed, 1 : 1,000) followed by peroxidase-conjugated secondary antibody (1 : 5,000) and enhanced chemiluminescence detection. Panels (b, d) show densitometric quantitation of replicate western blots.