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Inhibition of LPA receptor signaling blocks LPA-induced E-cadherin junction loss. OVCA429 cells were treated with LPA (80 μM) for 24 hours in the presence or absence of LPARI (20 μM) or DMSO (vehicle control), as indicated. (a) Conditioned media were examined by gelatin zymography. (b) Cells were processed for E-cadherin immunofluorescence using anti-E-cadherin ectodomain antibody (1 : 300) and Alexa Fluor 488-conjugated secondary antibody (1 : 500; green). Blue-DAPI-stained nuclei.
