Figure 7.

LPA treatment disrupts epithelial cohesion. Confluent layers of (a) OVCA429 and (b) OVCA433 cells were treated with LPA (40 μM) for 24 hours. Cells were then detached from substratum as cell-cell adherent sheets using dispase (1 mg/mL). Cohesive epithelial sheets were transferred to tubes then subjected to 50 rotations on a bench-top rocker returned to a 60 mm culture dish, and total fragment number was enumerated. Top panels show (a, b) OVCA429 and (c, d) OVCA433 control cells (BSA treated) as intact epithelial sheets and cohesive multicellular strands. LPA treatment of (e, f) OVCA429 and (g, h) OVCA433 cells decreases epithelial cohesion, as evidenced by increased fragment number enumerated in (I, J). *P < .02; **P < .002. Scale bar 1000 μm.