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. 2001 May 15;98(11):6342–6347. doi: 10.1073/pnas.111031498

Figure 4.

Figure 4

Proviral persistence and reactivation in prolonged cultures. (A) SupT1 cells infected with HIV-rtTA 2Δ tetO (Left) or HIV-rtTA 8 tetO (right) were kept in culture in the absence of dox for up to 6 months (experiment still in progress). Samples of the cultures were treated with dox on days 3, 15, 36, and 90, and virus production was measured by CA-p24 ELISA. (B) The average number of HIV proviral DNA molecules in the cells was monitored by competitive PCR. Values were normalized for the number of cellular diploid genomes as measured by competitive PCR on the cellular single-copy gene β-globin.