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. 2012 Apr 13;4(4):244–266. doi: 10.3390/toxins4040244

Table 1.

ELISA for OTA.

Year Antibody Format Matrix D.L. Validation Reference
1981 PAb 1 dc buffer 25 pg cr [69]
1988 MAb 1 ic barley 5 µg/kg [70]
1989 MAb 1 ic various 50 pg/mL + [71]
1990 MAb 1 dc barley 1 ng/mL + [72]
1991 MAb 1 dc barley 1 ng/mL + [73]
1996 MAb 1 dc cereals 0.5 ng/g + [74]
1996 MAb 1 dc buffer 45 pg/mL cr [75]
1997 MAb 1 dc serum 0.2 ng/mL + [34]
1998 MAb 1 dc plasma 4–20 pg/mL [38]
1999 MAb 2 m wheat 0.4 ng/mL +/− [76]
2000 MAb 1 ic chilies 5 ng/mLa + [77]
2002 MAb 1 dc plasma/tissue 0.5 ng/g + [43]
2002 MAb 2 m coffee 4 µg/kg cr [78]
2006 MAb 1 ic coffee 50 pg/mL + [28]
2007 MAb 1 ic coffee 3.73 ng/g + [79]
2007 PAb 1 m/dc chilies 10 µg/kg + [80]
2007 PAb 1 dc various 1 ng/mL +/− [81]
2009 MAb str corn 2.5 ng/mL [82]
2011 PAb 1 str grains 1.5 µg/kg + [83]
2011 MAb 1 ic cereals 1.70 ng/mL a [84]

1 = produced in-house; 2 = commercial; m = membrane; ic = indirect competitive; dc = direct competitive; str = strip test; a = IC50; cr = only cross-reactivity; + = validated; − = not validated; +/− = partially validated.