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RT-PCR of the assT-dsbL-dsbI gene cluster from S. Typhi IMSS-1 wild type with either the pFMTrc12 empty plasmid vector or the pFMTrcleuO-50 induced with 50 mM IPTG and from IMSS-1 STYhns99 in MA medium. As a gel loading control, a fragment of the gene encoding the 16S rRNA was amplified. As a full-length positive control, a PCR from genomic DNA was amplified.
