Fig 5.

assT promoter region in N-minimal medium. (A) Primer extension assay performed in N-minimal medium at 12 h from S. Typhi IMSS-1 wild type with pFMTrc12 and from IMSS-1 STYhns99, with both containing the assT−120/assT+93 gene fusion. (B) Upstream regulatory sequence of assT. The location of the PLeuO (−164) promoter observed in MA medium and the two transcription start sites, P1 (−31) and P2 (−36), observed in N-minimal medium are shown, as well the corresponding −10 TATA boxes, which are underlined. The initiation codon for assT is indicated in a rectangle.