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S. mutans multidrug tolerant persisters. During the antibiotic challenges, cells were removed at the indicated time points, serially diluted, and spot plated onto THYE agar plates for CFU determination. The S. mutans UA159 WT strain showed a biphasic killing curve for each of the antibiotics tested, where the majority of antibiotic-sensitive cells are rapidly killed, leaving intact a surviving subpopulation of persister cells.
