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Effect of CSP pheromone on the development of S. mutans persisters. Cells of UA159 WT strain and its quorum-sensing mutants (ΔcomC and ΔcomE) were preincubated with synthetic CSP (2 μM sCSP) for 2 h at 37°C before being challenged with ofloxacin at 20 μg/ml. Aliquots of cells were removed at the introduction of the antibiotic and after the antibiotic treatment (24 h) to determine cell survival by spot plating onto THYE agar plates.
