Table 1.

Germination of dormant and SD spores isolated using different nutrient germinants^a

Germinant	Spore germination in 2 h (%)
	Dormant	Superdormant, with nutrient used for SD spore isolation
		l-Valineb	AGFKb	l-Asparaginec
l-Valine	95	3	25	18
AGFK	93	22	7	NT
l-Asparagine	95	NT	NT	5

^aDormant and SD PS533 (wild-type) and FB10 (gerBB*) spores were isolated as described in Materials and Methods, with SD spores being prepared by germination with l-valine or AGFK (PS533 spores) or l-asparagine (FB10 spores). The dormant and SD spores were germinated with l-valine, AGFK, or l-asparagine, and the percentage of spores that germinated in 2 h was determined by phase-contrast microscopy as described in Materials and Methods. NT, not tested.

^bPS533 spores.

^cFB10 spores.