Table 3.

Relative levels of germination proteins in dormant and SD spores^a

Germination protein	Level in SD spores/level in dormant spores with GRs triggered to prepare SD sporesb
	GerAc	GerB + GerK	GerB*
GerAA	0.13d (0.16)	0.5e (0.5)	0.5e (0.47)
GerAC	0.17d (0.18)	0.5e (0.64)	0.4e (0.33)
GerBC	0.5d (0.63)	<0.1d,f (0.08)	<0.17d,f (0.12)
GerKA	0.6g (0.6)	0.13d (0.11)	0.5g (0.4)
SpoVAD	1.0 (1.1)	1.0 (0.99)	1.0 (0.96)
GerD	1.0 (1.0)	1.0 (0.8)	1.0 (0.92)

^aDormant and SD spores of strains PS533 (wild-type) (l-valine germination via the GerA GR and AGFK germination via the GerB and GerK GRs) and FB10 (gerBB*) (l-asparagine germination via the GerB* GR) were prepared, inner membrane fractions were isolated, and levels of germination proteins were determined as described in Materials and Methods and as shown in Fig. 1 to 3. Values shown are averages of results from two independent spore preparations, and these differed by ≤±15%.

^bValues were determined by visual inspection or by densitometric analysis using Image J (values in parentheses).

^cEssentially identical results were obtained with PS767 dormant and SD spores, with the SD spores being prepared by germination with l-valine via the GerA GR (data not shown).

^dP < 0.001.

^eP < 0.005.

^fThese numbers were estimated by visual inspection and extrapolation from the intensity of the band given by the lowest level of dormant spore inner membrane protein analyzed in parallel.

^gP < 0.1.