Table 1.
E. coli plasmids and strains
| Plasmid or strain | Description or genotypec | Reference and/or sourcea |
|---|---|---|
| Plasmids | ||
| pACYC184 | Catr Tetr | 23 |
| pBR322 | Tetr Ampr | 14 |
| pING1 | Ampr; paraBADaraC | 44 |
| pDS596 | Ampr; dnaA paraBAD | 43 |
| pLST435 M | Ampr; dnaA(T435M)paraBAD | 84 |
| pKC596 | Amprd; dnaAT7 gp10 promoter | 21 |
| pMMF41 | Catr; hda | 29 |
| pMMF83 | Catr; ralR rcbA ydaQ | This work |
| pMMF83-1 | Catr; ralR rcbA | This work |
| pMMF83-2 | Catr; ralR (ochre) rcbA | This work |
| pMMF83-3 | Catr; ralR (frameshift, ochre, opal) rcbA | This work |
| pMMF83-4 | Catr; ralR ydaQ | This work |
| pMMF85 | Catr; rcbA (CW) insert at BamHI site of pACYC184 | This work |
| pMMF86 | Catr; rcbA (CCW) insert at BamHI site of pACYC184 | This work |
| pMMF-D1 | Catr; rcbA insert at EcoRV site of pACYC184 | This work |
| pMMF-D4 | Catr; rcbA insert at EcoRV site of pACYC184 | This work |
| pMMF-D5 | Catr; rcbA insert at EcoRV site of pACYC184 | This work |
| pMMF-D13 | Catr; rcbA insert at EcoRV site of pACYC184 | This work |
| Strains | ||
| XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17(rK− mK+) supE44 relA1 lac [F′::Tn10 proAB+lacIqlacZΔM15] | Laboratory stock |
| JJC315 | leu6 his4 argE3 lacY1 galK2 ara14 xyl5 mtl1 tsx33 rpsL31 supE44 hsdR recB268::Tn10 | 67 |
| MC1061 | araD139 Δ(araleu)7697 ΔlacX74 galU galK rpsL hsdR2(rK− mK+) mcrB1 | Laboratory stock |
| SK002 | MC1061 recB268::Tn10 | 29 |
| MV1193 | Δ(lac-proAB) rpsL thi endA sbcB15 hsdR4 Δ(recA-srl)306::Tn10 [F′ traD36 proAB lacIqlacZΔM15] | Laboratory stock |
| HMS174 | (λDE3) recA1 hsdR(rK− mK+) Rifr | Laboratory stock |
| MG1655 | F− λ−rph-1 | Laboratory stock |
| JW1341-1b | Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ− ΔrcbA::kan rph-I Δ(rhaD-rhaB)568 hsdR514 | E. coli Genetic Stock Center; 5 |
| MF1341 | MG1655 ΔrcbA::kan | P1 (JW1341-1) × MG1655→Kanr; this work |
| N3793 | thr-1 araC14 leuB6(Am) Δ(gpt-proA)62 lacY1 tsx33 qsrr-0 glnV44(AS) galK2(Oc) λ−Rac-0 hisG4(Oc) rfbC1 mgl51 rpoS396(Am) rpsL3(Strr) kdgK51 xylA5 mtl-1 recG263(;;kan) argE3(Oc) thi-1 | E. coli Genetic Stock Center |
| JW5604-1 | Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ−rph-I Δrep-729::kan Δ(rhaD-rhaB)568 hsdR514 | E. coli Genetic Stock Center; 5 |
| MF1344 | MG1655 Δrep-729::kan | P1 (JW5604-1) × MG1655→Kanr; this work |
| MF0816 | MG1655 Δ(recA-srl)306::Tn10 | P1 (MV1193) × MG1655→Tetr; this work |
| MF0817 | MG1655 recB268::Tn10 | P1 (SK002) × MG1655→Τetr; this work |
| MF1342 | MF1341 Δ(recA-srl)306::Tn10 | P1 (MV1193) × MF1341→Tetr; this work |
| MF1343 | MF1341 recB268::Tn10 | P1 (SK002) × MF1341→Tetr; this work |
| SS1020 | F− λ−rph-1 zjj202::Tn10 dnaC2 | Steven Sandler |
| MF1345 | MG1655 zjj202::Tn10 dnaC2 | P1 (SS1020) × MG1655→Tetr; this work |
| MF1346 | MF1341 zjj202::Tn10 dnaC2 | P1 (SS1020) × MF1341→Tetr; this work |
a
For strains, the arrows indicate the method of selection after transduction with the P1 bacteriophage.
b
The ΔrcbA::kan mutation is identical to the ΔydaC784::kan mutation in JW1341-1.
c
CW, clockwise; CCW, counterclockwise.
d
See reference 43.