Table 1.
Primer designation | Sequence (5′-3′)a | T. pallidum Nichols genome coordinates |
---|---|---|
TprCFL (5′) | TTTTGGATCCCATGGGCGTACTCACTCCG | 134982-134967 |
TprCFL (3′) | GCCCAAGCTTCCATGTCACTTTCATTCCGCA | 136673-136697 |
TprCSp (5′) | ACCGGATCCCATGAGAGCTATCCTCAAAGCA | 135789-135771 |
TprCSp (3′) | GCGCTAAGCTTGACAACTCTTGGATCGGA | 136044-136062 |
TprCNT (5′) | GATAGAATTCTATGGGGGTGTGGGCACAGCTGCAG | 135167-135147 |
TprCNT (3′) | GATAAAGCTTACTCTGGTGTTGGTTACCGGCGTCGA | 135744-134770 |
TprCCT (5′) | GATAGAATTCCATGCTCAACATAGACGCGCTCCTGC | 136114-136092 |
TprCCT (3′) | GATCAAGCTTCCATGTCACTTTCATTCCGCAGA | 136671-136694 |
TprC (5′) (Cali-77 amplification) | CAAGAGAAGGCGAGTGTGTAGGTGCTCATG | 134830-134801 |
TprC (3′) (Cali-77 amplification) | GAAGAGGCAGCCCTCATCCGAGACAAA | 136769-136797 |
Underlined sequences indicate restriction sites.