Table 1.
Primers used in this study
| Primer designation | Sequence (5′-3′)a | T. pallidum Nichols genome coordinates |
|---|---|---|
| TprCFL (5′) | TTTTGGATCCCATGGGCGTACTCACTCCG | 134982-134967 |
| TprCFL (3′) | GCCCAAGCTTCCATGTCACTTTCATTCCGCA | 136673-136697 |
| TprCSp (5′) | ACCGGATCCCATGAGAGCTATCCTCAAAGCA | 135789-135771 |
| TprCSp (3′) | GCGCTAAGCTTGACAACTCTTGGATCGGA | 136044-136062 |
| TprCNT (5′) | GATAGAATTCTATGGGGGTGTGGGCACAGCTGCAG | 135167-135147 |
| TprCNT (3′) | GATAAAGCTTACTCTGGTGTTGGTTACCGGCGTCGA | 135744-134770 |
| TprCCT (5′) | GATAGAATTCCATGCTCAACATAGACGCGCTCCTGC | 136114-136092 |
| TprCCT (3′) | GATCAAGCTTCCATGTCACTTTCATTCCGCAGA | 136671-136694 |
| TprC (5′) (Cali-77 amplification) | CAAGAGAAGGCGAGTGTGTAGGTGCTCATG | 134830-134801 |
| TprC (3′) (Cali-77 amplification) | GAAGAGGCAGCCCTCATCCGAGACAAA | 136769-136797 |
a
Underlined sequences indicate restriction sites.