Fig 1.

RT-PCR analysis of transcripts from S. coelicolor M145 (parental strain) and JSE1880 (rnc-null mutant). RT-PCR was performed as described previously (8, 16), and products were analyzed after 20 PCR cycles to ensure linearity between band intensity and the number of cycles. Lane 1, size standards (the arrow indicates the 500-bp standard); lane 2, mRNA from M145 as the template for reverse transcription; lane 3, M145 BARD RNA; lane 4, M145 mock BARD RNA; lanes 5 and 8, JSE1880 mRNA; lanes 6 and 9, JSE1880 BARD RNA; lanes 7 and 10, JSE1880, mock BARD RNA. The primers used for the PCRs for lanes 2 to 7 were specific for the readthrough transcript from the S. coelicolor rpsO-pnp operon and produced a 460-bp fragment, while the primers used for lanes 8 to 10 were specific for ramR, producing a 583-bp fragment.