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Outline of the RNA immunoprecipitation (RIP) procedure. As described in detail in Materials and Methods, the procedure involved binding the RNase III mutant protein, RNase III D70A, to S. coelicolor mRNA, binding anti-RNase III D70A antibody to the RNA-RNase III D70A complexes, and isolation of the antibody-RNA-RNase III D70A complexes using magnetic beads.
