Table 3.

Comparison of RNA-Seq and microarray analyses of parental and mutant strains of Streptomyces coelicolor

Transcript	Fold increase or decreasea	Protein encoded by the gene	Total no.	Present in BARD
Transcripts whose levels changed by ≥2-fold in the RNA-seq analysis and by ≥6-fold in the microarray analysis
SCO3142	2.23	Conserved hypothetical protein		No
SCO3152	2.73	Conserved hypothetical protein		Yes
SCO3216	−4.81	Membrane ATPase		No
SCO3217	−2.18	CdaR		No
SCO3223	−13.76	Putative ABC transporter		No
SCO3224	−9.71	Putative ABC transporter ATP-binding protein		No
SCO3236	−3.02	Possible antibiotic oxygenase		No
SCO3244	−4.19	Putative secreted protein		No
SCO3984	2.23	Conserved hypothetical protein		Yes
SCO4843	2.20	Putative integral membrane protein		No
SCO4880	3.91	Possible transferase		Yes
SCO4947	3.23	Nitrate reductase α chain, NarG3		Yes
SCO4948	3.77	Nitrate reductase β chain, NarH3		Yes
SCO5107	3.16	Putative succinate dehydrogenase membrane subunit		Yes
SCO6276	−4.89	Epoxide hydrolase		No
SCO6277	−4.95	Putative secreted protein		Yes
SCO6278	−4.06	Integral membrane transport protein		No
SCO6280	−2.00	Similar to Streptomyces nogalater nogalamycin biosynthesis activator, SnoA		No
SCO6282	−15.73	Probable 3-oxoacyl-[acyl-carrier protein] reductase		Yes
SCO6283	−2.56	Conserved hypothetical protein		No
SCO6682	−3.80	Conserved hypothetical protein	21	No
Transcripts whose levels changed by ≥2-fold in the RNA-Seq analysis and by <6-fold in the microarray analysis			51
Transcripts whose levels changed by ≥2-fold in the RNA-Seq analysis but which were not listed in the microarray analysis			72
Transcripts whose levels changed by ≥6-fold in the microarray analysis but by <2-fold in the RNA-Seq analysis			165

^aFold increase or decrease in the level of a given transcript in JSE1880 as compared with that of M145.