Fig 2.

(A) The visual appearance of the BU LAMP amplification product after addition of a 1:10 dilution of SYBR green I dye. The dye fluoresces strongly when bound to the double-stranded DNA, and the resulting DNA-dye complex gives a green color, while fluorescence is minimal when the dye is free in the solution and gives an orange/brown color. (B) The amplification curves obtained using the ESE-Quant tube scanner. Results can be read using the LCD panel as either positive or negative and/or in real time using a computer with the appropriate software (9). The graph reports the fluorescence in millivolts (mV) on the y axis and time in minutes on the x axis. Sample 1, M. ulcerans-spiked soil; 2, supernatant from positive specimen; 3, DNA from needle aspirate; 4, M. marinum; control (C), M. ulcerans DNA (Agy99); negative control (NC), water.