Fig 1.

A TRCR method for the detection of EV71 from clinical specimens. (A) Procedure and a primer-probe set for the TRCR method. Viral RNA and TRCR reagents and enzymes were preincubated at 43°C for 5 min and 2 min, respectively, and then were mixed. EV71-targeting regions and T7 promoter regions of the forward primers are colored red and blue, respectively. The positions of oxazole yellow dye in INAF probes are indicated by green asterisks. (B) Histogram of RNA samples extracted from throat swabs of HFMD or herpangina patients in Japan (total 58 samples) grouped by the numbers of copies of viral RNA.