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Assessment of detection sensitivity of the “most promising” coated beads, used singly (A) or as 50:50 mixes (B), for capture of M. bovis AF2122/97 from spiked broth suspensions containing 10 to 105 CFU/ml by IMS-PCR. The expected IS6110 touchdown PCR product size was 245 bp. The DNA ladder shows X174 RF DNA/HaeIII fragments (Life Technologies).
