Table 3.
Outcomes of phage display biopanning of whole M. bovis cells (WCA), ethanol-extracted M. bovis cell surface antigens (EEA), and M. bovis surface antigen MPB83a
| Antigen | Clone identifier(s) | Amino acid sequence | Frequencyb | Ability to bind WCA |
|---|---|---|---|---|
| WCA | 302, 305, 309 | FHWTWQFPYTST | 3/10 | No |
| 303, 304, 307 | FHWNYYLYSQVS | 3/10 | No | |
| 301, 306 | WHWQWWLQTDAT | 2/10 | No | |
| 308 | FHWWPPSLANQP | 1/10 | No | |
| 310 | WHWNAWNWSSQQ | 1/10 | No | |
| EEA | 301, 303, 304, 306, 307, 308, 309 | SEFPRSWDMETN | 7/10 | Yes |
| 305 | FEFPRSWDMETN | 1/10 | No | |
| 302 | NFRVSIDVVKSR | 1/10 | Yes | |
| 310 | Unsuccessful sequencing | 1/10 | ||
| MPB83 | 304, 307 | GNLLHNHETYRH | 2/10 | Noc |
| 305 | HSLRWDWTARNS | 1/10 | Noc | |
| 306 | HSLRDDIRTMTA | 1/10 | Noc | |
| 309 | HKWGGNTLMAFR | 1/10 | Not tested | |
| 310 | HKPPTHIYLSWR | 1/10 | Not tested | |
| 308 | KVWPNMFANENI | 1/10 | Not tested | |
| 302 | KLWSIPKDLGPP | 1/10 | Not tested | |
| 303 | GYVHRELAWNMN | 1/10 | Not tested | |
| 301 | NMELHPHSLPRP | 1/10 | Not tested |
a
Two EEA-derived peptides (highlighted in bold) capable of binding WCA were chemically synthesized and subsequently evaluated for IMS purposes.
b
Number of clones with sequence/total number of clones tested after 3rd round of biopanning.
c
Did not bind WCA but did bind MPB83.