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Organization of the pha locus in Cupriavidus sp. S-6 and construction of the artificial pha operon and chimeric phaC genes. (A) Organization of the phaCCsp, phaACsp, and phaBCsp genes in the pha operon from Cupriavidus sp. S-6 and restriction map of the 9-kb EcoRI-cloned DNA fragment. (B) Construction of the artificial pha operon. (C) Construction of chimeric phaC genes. Details of the construction of the artificial pha operon and chimeric phaC are described in Materials and Methods.
