Table 1.
Primers used in this study
| Primer | Sequencea |
|---|---|
| EcoRI23SDF | 5′-CGGAATTCGAAGGAGATATACATATG-3′ |
| 23BamHIR | 5′-AGCGGATCCTGGTGCTCGAGTGC-3′ |
| CspCNdeIF | 5′-AGAGACACGACCATATGGCGACCGGC-3′ |
| CspCHindIIIR | 5′-CCGGAAGCTTAAGCCTTGGCCTTGA-3′ |
| CspAB–BamHIF | 5′-CAAGGATCCCAACCGTTACCGAAGGC-3′ |
| CspAB–NotIR | 5′-ATAGTTTAGCGGCCGCTCGGTCAGCCCATGTG-3′ |
| H16AB–BamHIF | 5′-TCCCTCCGGATCCCATTGAAAGGACT-3′ |
| H16AB–NotIR | 5′-TGAACCAGGGCGGCCGCTCAGCCCATAT-3′ |
| CspC505F | 5′-GATGCGATGTCGCCGGCCAATTTCCT-3′ |
| CspC530R | 5′-AGGAAATTGGCCGGCGACATCGCATC-3′ |
| CspC790F | 5′-AACAAGTACTACATCCTCGACCTGCAG-3′ |
| CspC816R | 5′-CTGCAGGTCGAGGATGTAGTACTTGTT-3′ |
| CspC1253F | 5′-TGGTCTGGAACTACGTGGTCGACAACT-3′ |
| CspC1279R | 5′-AGTTGTCGACCACGTAGTTCCAGACCA-3′ |
| CspC1582F | 5′-GTCATCAATCCGCCGGCGAAGAACAA-3′ |
| CspC1607R | 5′-TTGTTCTTCGCCGGCGGATTGATGAC-3′ |
| PhaCF1 | 5′-ATCAACAAGTWCTACRTCYTSGACCT-3′ |
| PhaCR2 | 5′-GTSTTCRTSRTSWSCTGGCGCAACCC-3′ |
| G286AF | 5′-ACGCCAGCATGGCCGCGAGCACCTGGGACG-3′ |
| G286AR | 5′-CGTCCCAGGTGCTCGCGGCCATGCTGGCGT-3′ |
| A341QF | 5′-GCGAGCACCCGGCCCAGAGCGTCACGCTGC-3′ |
| A341QR | 5′-GCAGCGTGACGCTCTGGGCCGGGTGCTCGC-3′ |
a
The sequence recognized by the restriction enzyme is underlined; the ribosome binding site sequence is double underlined. The sequences of CspC530R, CspC816R, CspC1279R, and Csp1607R are complementary to those of CspC505F, CspC790F, CspC1253F, and CspC1582F, respectively. Gray shading indicates the mutation site.